GINS2
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
CDC45
Gene Ontology Biological Process
- DNA duplex unwinding [IBA]
- DNA replication [TAS]
- DNA replication checkpoint [TAS]
- DNA strand elongation involved in DNA replication [TAS]
- G1/S transition of mitotic cell cycle [TAS]
- double-strand break repair via break-induced replication [IBA]
- mitotic DNA replication preinitiation complex assembly [IBA]
- mitotic cell cycle [TAS]
- positive regulation of G1/S transition of mitotic cell cycle [IBA]
- pre-replicative complex assembly involved in nuclear cell cycle DNA replication [IBA]
- regulation of chromatin silencing at telomere [IBA]
- regulation of transcription involved in G1/S transition of mitotic cell cycle [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Protein interaction and cellular localization of human CDC45.
CDC45, which plays a role in eukaryotic DNA replication, is a member of the CMG (CDC45/MCM2-7/GINS) complex that is thought to function as a replicative DNA helicase. However, the biochemical properties of CDC45 are not fully understood. We systematically examined the interactions of human CDC45 with MCM2-7, GINS and other replication proteins by immunoprecipitation. We found that CDC45 can directly ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| CDC45 GINS2 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 1 | BioGRID | 3176749 | |
| GINS2 CDC45 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.9998 | BioGRID | 3094904 |
Curated By
- BioGRID