BAIT

TPL

F7H2.9, F7H2_9, TOPLESS, WSIP1, WUS-INTERACTING PROTEIN 1, AT1G15750
protein TOPLESS
Arabidopsis thaliana (Columbia)
PREY

IAA13

F4P9.8, F4P9_8, auxin-induced protein 13, AT2G33310
auxin-responsive protein IAA13
GO Process (1)
GO Function (2)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

A user-friendly platform for yeast two-hybrid library screening using next generation sequencing.

Erffelinck ML, Ribeiro B, Perassolo M, Pauwels L, Pollier J, Storme V, Goossens A

Yeast two-hybrid (Y2H) is a well-established genetics-based system that uses yeast to selectively display binary protein-protein interactions (PPIs). To meet the current need to unravel complex PPI networks, several adaptations have been made to establish medium- to high-throughput Y2H screening platforms, with several having successfully incorporated the use of the next-generation sequencing (NGS) technology to increase the scale and sensitivity ... [more]

PLoS One Dec. 24, 2018; 13(12);e0201270 [Pubmed: 30576311]

Throughput

  • High Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
TPL IAA13
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID