BAIT
TPL
F7H2.9, F7H2_9, TOPLESS, WSIP1, WUS-INTERACTING PROTEIN 1, AT1G15750
protein TOPLESS
GO Process (5)
GO Function (2)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Arabidopsis thaliana (Columbia)
PREY
ZFP3
F21J6.6, F21J6_6, zinc finger protein 3, AT5G25160
zinc finger protein 3
GO Process (1)
GO Function (4)
GO Component (1)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Arabidopsis thaliana (Columbia)
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
A user-friendly platform for yeast two-hybrid library screening using next generation sequencing.
Yeast two-hybrid (Y2H) is a well-established genetics-based system that uses yeast to selectively display binary protein-protein interactions (PPIs). To meet the current need to unravel complex PPI networks, several adaptations have been made to establish medium- to high-throughput Y2H screening platforms, with several having successfully incorporated the use of the next-generation sequencing (NGS) technology to increase the scale and sensitivity ... [more]
PLoS One Dec. 24, 2018; 13(12);e0201270 [Pubmed: 30576311]
Throughput
- High Throughput
Curated By
- BioGRID