UBB
Gene Ontology Biological Process
- adipose tissue development [IMP]
- energy homeostasis [IMP]
- fat pad development [IMP]
- female gonad development [IMP]
- female meiosis I [IMP]
- hypothalamus gonadotrophin-releasing hormone neuron development [IMP]
- male gonad development [IMP]
- male meiosis I [IMP]
- mitochondrion transport along microtubule [ISO]
- neuron projection morphogenesis [ISO]
- positive regulation of intrinsic apoptotic signaling pathway by p53 class mediator [ISO]
- regulation of mitochondrial membrane potential [ISO]
- regulation of neuron death [ISO]
- regulation of proteasomal protein catabolic process [ISO]
- seminiferous tubule development [IMP]
- ubiquitin homeostasis [IGI, IMP]
Gene Ontology Cellular Component
POLK
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- nucleoplasm [ISO]
- nucleus [IDA]
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
Requirements for the interaction of mouse Polkappa with ubiquitin and its biological significance.
Polkappa protein is a eukaryotic member of the DinB/Polkappa branch of the Y-family DNA polymerases, which are involved in the tolerance of DNA damage by replicative bypass. Despite universal conservation through evolution, the precise role(s) of Polkappa in this process has remained unknown. Here we report that mouse Polkappa can physically interact with ubiquitin by yeast two-hybrid screening, glutathione S-transferase ... [more]
Throughput
- Low Throughput
Additional Notes
- #LPPI
- Likely protein-protein interaction
Curated By
- BioGRID