PREY

RIPK1

D330015H01Rik, RIP, Rinp, Rip1
receptor (TNFRSF)-interacting serine-threonine kinase 1
GO Process (37)
GO Function (8)
GO Component (7)

Gene Ontology Biological Process

Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Regulation of early wave of germ cell apoptosis and spermatogenesis by deubiquitinating enzyme CYLD.

Wright A, Reiley WW, Chang M, Jin W, Lee AJ, Zhang M, Sun SC

Spermatogenesis involves an early wave of germ cell apoptosis, which is required for maintaining the balance between germ cells and supporting Sertoli cells. However, the signaling mechanism regulating this apoptotic event is poorly defined. Here we show that genetic deficiency of Cyld, a recently identified deubiquitinating enzyme, attenuates the early wave of germ cell apoptosis and causes impaired spermatogenesis in ... [more]

Dev. Cell Nov. 01, 2007; 13(5);705-16 [Pubmed: 17981138]

Throughput

  • Low Throughput

Curated By

  • BioGRID