An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

UHRF2 commissions the completion of DNA demethylation through allosteric activation by 5hmC and K33-linked ubiquitination of XRCC1.

Liu X, Xu B, Yang J, He L, Zhang Z, Cheng X, Yu H, Liu X, Jin T, Peng Y, Huang Y, Xia L, Wang Y, Wu J, Wu X, Liu S, Shan L, Yang X, Sun L, Liang J, Zhang Y, Shang Y

The transition of oxidized 5-methylcytosine (5mC) intermediates into the base excision repair (BER) pipeline to complete DNA demethylation remains enigmatic. We report here that UHRF2, the only paralog of UHRF1 in mammals that fails to rescue Uhrf1-/- phenotype, is physically and functionally associated with BER complex. We show that UHRF2 is allosterically activated by 5-hydroxymethylcytosine (5hmC) and acts as a ... [more]

Mol Cell Dec. 15, 2020; 81(14);2960-2974.e7 [Pubmed: 34111398]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

TDG

Gene Ontology Biological Process

Gene Ontology Molecular Function

DNA N-glycosylase activity [IDA]damaged DNA binding [TAS]double-stranded DNA binding [IDA]mismatched DNA binding [IDA]protein binding [IPI]protein homodimerization activity [IDA]pyrimidine-specific mismatch base pair DNA N-glycosylase activity [IDA]structure-specific DNA binding [ISS]

Gene Ontology Cellular Component

XRCC1