Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

[Screening of interacting proteins of idiopathic gonadotropin-releasing hormone deficiency pathogenic gene RNF216].

Dai W, Jiang Z, Gu M, Zhu Y, Tang M, Chen X

To screen proteins interacting with ring finger protein 216(RNF216) through yeast two hybrid experiment, and further clarify the role of RNF216 in the pathogenesis of gonadotropin-releasing hormone deficiency.A recombinant expression vector pGBKT7-RNF216 was constructed and transformed into yeast Y2HGold, which was hybridized with a human cDNA library in order to screen proteins interacting with RNF216. The interaction was verified in ... [more]

Zhonghua Yi Xue Yi Chuan Xue Za Zhi Jul. 10, 2021; 38(7);631-634 [Pubmed: 34247365]

Throughput

  • Low Throughput

Curated By

  • BioGRID