BAIT

MAPT

AI413597, AW045860, Mtapt, Tau, RP23-136D4.1
microtubule-associated protein tau
Mus musculus
PREY

HSPA8

2410008N15Rik, Hsc70, Hsc71, Hsc73, Hsp73, Hspa10
heat shock protein 8
Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

BAG-1 associates with Hsc70.Tau complex and regulates the proteasomal degradation of Tau protein.

Elliott E, Tsvetkov P, Ginzburg I

Intraneuronal accumulation of phosphorylated Tau protein is a molecular pathology found in many forms of dementia, including Alzheimer disease. Research into possible mechanisms leading to the accumulation of modified Tau protein and the possibility of removing Tau protein from the system have revealed that the chaperone protein system can interact with Tau and mediate its degradation. Hsp70/Hsc70, a member of ... [more]

J. Biol. Chem. Dec. 21, 2007; 282(51);37276-84 [Pubmed: 17954934]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
MAPT HSPA8
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Low-BioGRID
-
MAPT HSPA8
Co-fractionation
Co-fractionation

Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.

High0.9085BioGRID
2670119

Curated By

  • BioGRID