An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Kv2 channel-AMIGO ?-subunit assembly modulates both channel function and cell adhesion molecule surface trafficking.

Maverick EE, Leek AN, Tamkun MM

The Kv2 channels encode delayed rectifier currents that regulate membrane potential in many tissues. They also have a non-conducting function to form stable junctions between the endoplasmic reticulum and plasma membranes, creating membrane contact sites that mediate functions distinct from membrane excitability. Therefore, proteins that interact with Kv2.1 and Kv2.2 channels can alter conducting and/or non-conducting channel properties. One member ... [more]

J Cell Sci Dec. 15, 2020; 134(12); [Pubmed: 34137443]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

KCNB1

Gene Ontology Biological Process

Gene Ontology Molecular Function

delayed rectifier potassium channel activity [IBA]protein binding [IPI]

Gene Ontology Cellular Component

AMIGO1