Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Proteome changes induced by knock-down of the deubiquitylating enzyme HAUSP/USP7.

Kessler BM, Fortunati E, Melis M, Pals CE, Clevers H, Maurice MM

Modification of proteins by ubiquitin plays a major role in a broad array of biological processes. Reversal of this process through deubiquitylation likely represents an important regulatory step in the maintenance of cellular homeostasis. However, the biological functions of deubiquitylating enzymes still remain poorly characterized. To investigate the biological role of the herpes virus-associated ubiquitin-specific protease HAUSP/USP7, we have generated ... [more]

J. Proteome Res. Nov. 01, 2007; 6(11);4163-72 [Pubmed: 17927229]

Throughput

  • Low Throughput

Additional Notes

  • not confirmed human

Curated By

  • BioGRID