An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Quantitative dissection and stoichiometry determination of the human SET1/MLL histone methyltransferase complexes.

van Nuland R, Smits AH, Pallaki P, Jansen PW, Vermeulen M, Timmers HT

Methylation of lysine 4 on histone H3 (H3K4) at promoters is tightly linked to transcriptional regulation in human cells. At least six different COMPASS-like multisubunit (SET1/MLL) complexes that contain methyltransferase activity for H3K4 have been described, but a comprehensive and quantitative analysis of these SET1/MLL complexes is lacking. We applied label-free quantitative mass spectrometry to determine the subunit composition and ... [more]

Mol Cell Biol May. 01, 2013; 33(10);2067-77 [Pubmed: 23508102]

Throughput

High Throughput

Curated By

BioGRID

Interaction Summary

PSIP1

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase II transcription coactivator activity [IDA]activating transcription factor binding [IDA]chromatin binding [IDA]poly(A) RNA binding [IDA]protein binding [IPI]supercoiled DNA binding [ISS]

Gene Ontology Cellular Component

LDHB