BAIT

WDR82

9430077D24Rik, CDW5/WDR82
WD repeat domain containing 82
GO Process (1)
GO Function (2)
GO Component (5)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Mus musculus
PREY

EEF1A2

EEF1AL, EF-1-alpha-2, EF1A, HS1, STN, STNL
eukaryotic translation elongation factor 1 alpha 2
GO Process (0)
GO Function (1)
GO Component (0)

Gene Ontology Molecular Function

Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Quantitative dissection and stoichiometry determination of the human SET1/MLL histone methyltransferase complexes.

van Nuland R, Smits AH, Pallaki P, Jansen PW, Vermeulen M, Timmers HT

Methylation of lysine 4 on histone H3 (H3K4) at promoters is tightly linked to transcriptional regulation in human cells. At least six different COMPASS-like multisubunit (SET1/MLL) complexes that contain methyltransferase activity for H3K4 have been described, but a comprehensive and quantitative analysis of these SET1/MLL complexes is lacking. We applied label-free quantitative mass spectrometry to determine the subunit composition and ... [more]

Mol Cell Biol May. 01, 2013; 33(10);2067-77 [Pubmed: 23508102]

Throughput

  • High Throughput

Curated By

  • BioGRID