Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Proteome analysis of the circadian clock protein PERIOD2.

Gul H, Selvi S, Yilmaz F, Ozcelik G, Olfaz-Aslan S, Yazan S, Tiryaki B, Gul S, Yurtseven A, Kavakli IH, Ozlu N, Ozturk N

Circadian rhythms are a series of endogenous autonomous 24-hour oscillations generated by the circadian clock. At the molecular level, the circadian clock is based on a transcription-translation feedback loop, in which BMAL1 and CLOCK transcription factors of the positive arm activate the expression of CRYPTOCHROME (CRY) and PERIOD (PER) genes of the negative arm as well as the circadian clock-regulated ... [more]

Proteins Feb. 04, 2022; (); [Pubmed: 35122331]

Throughput

  • High Throughput

Additional Notes

  • BioID
  • Time point: 36 hours
  • Time point: 48 hours
  • Used SAINT as quantitative method

Curated By

  • BioGRID