Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Opposite regulation of oligodendrocyte apoptosis by JNK3 and Pin1 after spinal cord injury.

Li QM, Tep C, Yune TY, Zhou XZ, Uchida T, Lu KP, Yoon SO

Although oligodendrocytes undergo apoptosis after spinal cord injury, molecular mechanisms responsible for their death have been unknown. We report that oligodendrocyte apoptosis is regulated oppositely by c-Jun N-terminal kinase 3 (JNK3) and protein interacting with the mitotic kinase, never in mitosis A I (Pin1), the actions of which converge on myeloid cell leukemia sequence-1 (Mcl-1). Activated after injury, JNK3 induces ... [more]

J. Neurosci. Aug. 01, 2007; 27(31);8395-404 [Pubmed: 17670986]

Throughput

  • Low Throughput

Curated By

  • BioGRID