BAIT

CPSF100

ATCPSF100, CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR, CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR 100, EMB1265, EMBRYO DEFECTIVE 1265, ENHANCED SILENCING PHENOTYPE 5, ESP5, AT5G23880
cleavage and polyadenylation specificity factor 100
GO Process (4)
GO Function (2)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)
PREY

NPAP

PAP(IV), nuclear poly(a) polymerase, poly(A) polymerase IV, AT4G32850
nuclear poly(a) polymerase
GO Process (1)
GO Function (2)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

An interaction between an Arabidopsis poly(A) polymerase and a homologue of the 100 kDa subunit of CPSF.

Elliott BJ, Dattaroy T, Meeks-Midkiff LR, Forbes KP, Hunt AG

The Arabidopsis genome possesses a number of sequences that are predicted to encode proteins that are similar to mammalian and yeast polyadenylation factor subunits. One of these resides on chromosome V and has the potential to encode a polypeptide related to the 100 kDa subunit of the mammalian cleavage and polyadenylation specificity factor (CPSF). This gene encodes a ca. 2400 ... [more]

Plant Mol. Biol. Feb. 01, 2003; 51(3);373-84 [Pubmed: 12602868]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
NPAP CPSF100
Reconstituted Complex
Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Low-BioGRID
-

Curated By

  • BioGRID