BAIT

PP2CA

AHG3, ARABIDOPSIS THALIANA PROTEIN PHOSPHATASE 2CA, ATPP2CA, protein phosphatase 2CA, AT3G11410
protein phosphatase 2CA
Arabidopsis thaliana (Columbia)
PREY

KT2/3

AKT2, AKT2/3, AKT3, T10I14.30, T10I14_30, potassium transport 2/3, AT4G22200
potassium channel AKT2/3
GO Process (2)
GO Function (4)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Physical and functional interaction of the Arabidopsis K(+) channel AKT2 and phosphatase AtPP2CA.

Cherel I, Michard E, Platet N, Mouline K, Alcon C, Sentenac H, Thibaud JB

The AKT2 K(+) channel is endowed with unique functional properties, being the only weak inward rectifier characterized to date in Arabidopsis. The gene is expressed widely, mainly in the phloem but also at lower levels in leaf epiderm, mesophyll, and guard cells. The AKT2 mRNA level is upregulated by abscisic acid. By screening a two-hybrid cDNA library, we isolated a ... [more]

Plant Cell May. 01, 2002; 14(5);1133-46 [Pubmed: 12034902]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
KT2/3 PP2CA
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-
PP2CA KT2/3
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID