CSN5B
Gene Ontology Biological Process
- COP9 signalosome assembly [IMP]
- negative regulation of photomorphogenesis [IGI]
- photomorphogenesis [TAS]
- positive regulation of G2/M transition of mitotic cell cycle [IMP]
- positive regulation of protein catabolic process [IMP]
- protein deneddylation [IGI]
- regulation of L-ascorbic acid biosynthetic process [IMP]
- response to auxin [IGI]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- COP9 signalosome [IPI, TAS]
- nucleus [ISM]
CSN6A
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- COP9 signalosome [IPI, TAS]
- nucleus [TAS]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
The Arabidopsis CSN5A and CSN5B subunits are present in distinct COP9 signalosome complexes, and mutations in their JAMM domains exhibit differential dominant negative effects on development.
The COP9 signalosome (CSN) is an evolutionarily conserved multisubunit protein complex involved in a variety of signaling and developmental processes through the regulation of protein ubiquitination and degradation. A known biochemical role attributed to CSN is a metalloprotease activity responsible for the derubylation of cullins, core components for several types of ubiquitin E3 ligases. The CSN's derubylation catalytic center resides ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| CSN6A CSN5B | Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex. | High | 0.8544 | BioGRID | 2913695 | |
| CSN5B CSN6A | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID