An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Human transcription factor protein interaction networks

Goos H, Kinnunen M, Salokas K, Tan Z, Liu X, Yadav L, Zhang Q, Wei G-H, Varjosalo M

Transcription factors (TFs) interact with several other proteins in the process of transcriptional regulation. Here, we identify 6703 and 1536 protein-protein interactions for 109 different human TFs through proximity-dependent biotinylation (BioID) and affinity purification mass spectrometry (AP-MS), respectively. The BioID analysis identifies more high-confidence interactions, highlighting the transient and dynamic nature of many of the TF interactions. By performing clustering ... [more]

Nature Communications Feb. 09, 2022; (); [Pubmed: 35140242]

Throughput

High Throughput

Additional Notes

BioID

Curated By

BioGRID

Interaction Summary

TAL1

Gene Ontology Biological Process

Gene Ontology Molecular Function

E-box binding [IDA]RNA polymerase II transcription factor binding [IPI]chromatin binding [IDA]enzyme binding [IPI]histone deacetylase binding [IPI]protein binding [IPI]sequence-specific DNA binding transcription factor activity [IDA]transcription regulatory region DNA binding [ISS]

Gene Ontology Cellular Component

DBT