An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Human transcription factor protein interaction networks

Goos H, Kinnunen M, Salokas K, Tan Z, Liu X, Yadav L, Zhang Q, Wei G-H, Varjosalo M

Transcription factors (TFs) interact with several other proteins in the process of transcriptional regulation. Here, we identify 6703 and 1536 protein-protein interactions for 109 different human TFs through proximity-dependent biotinylation (BioID) and affinity purification mass spectrometry (AP-MS), respectively. The BioID analysis identifies more high-confidence interactions, highlighting the transient and dynamic nature of many of the TF interactions. By performing clustering ... [more]

Nature Communications Feb. 09, 2022; (); [Pubmed: 35140242]

Throughput

High Throughput

Additional Notes

Filtered using SAINT software tools (cutoff 0.74) and CRAPome database

Curated By

BioGRID

Interaction Summary

KLF15

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IC]protein binding [IPI]transcription regulatory region DNA binding [ISS]

Gene Ontology Cellular Component

ZNF850

Affinity Capture-MS

Publication

Human transcription factor protein interaction networks

Throughput

Additional Notes

Curated By

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IC]
protein binding [IPI]
transcription regulatory region DNA binding [ISS]