BAIT

KLF8

BKLF3, ZNF741, RP13-1021K9.1
Kruppel-like factor 8
GO Process (0)
GO Function (0)
GO Component (0)
Homo sapiens
PREY

VAPA

VAP-33, VAP-A, VAP33, hVAP-33
VAMP (vesicle-associated membrane protein)-associated protein A, 33kDa
Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Human transcription factor protein interaction networks

Goos H, Kinnunen M, Salokas K, Tan Z, Liu X, Yadav L, Zhang Q, Wei G-H, Varjosalo M

Transcription factors (TFs) interact with several other proteins in the process of transcriptional regulation. Here, we identify 6703 and 1536 protein-protein interactions for 109 different human TFs through proximity-dependent biotinylation (BioID) and affinity purification mass spectrometry (AP-MS), respectively. The BioID analysis identifies more high-confidence interactions, highlighting the transient and dynamic nature of many of the TF interactions. By performing clustering ... [more]

Nature Communications Feb. 09, 2022; (); [Pubmed: 35140242]

Throughput

  • High Throughput

Additional Notes

  • Filtered using SAINT software tools (cutoff 0.74) and CRAPome database

Curated By

  • BioGRID