An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Cytoplasmic RAD23B interacts with CORO1C to synergistically promote colorectal cancer progression and metastasis.

Li J, Tian L, Jing Z, Guo Z, Nan P, Liu F, Zou S, Yang L, Xie X, Zhu Y, Zhao Y, Sun W, Sun Y, Zhao X

Colorectal cancers (CRCs) are characterized by diffuse in?ltration of tumor cells into the regional lymph nodes and metastasis to distant organs, and its highly invasive nature contributes to disease recurrence and poor outcomes. The molecular mechanisms underlying CRC cell invasion remain incompletely understood. Here, we identi?ed the upregulation of DNA damage repair-related protein RAD23B in CRC cells and tissues and ... [more]

Cancer Lett Dec. 28, 2020; 516();13-27 [Pubmed: 34062216]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
CORO1C RAD23B	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Li J (2021)	Low	-	BioGRID	-
RAD23B CORO1C	Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.	Wan C (2015)	High	0.0953	BioGRID	1260340

Curated By

BioGRID

Interaction Summary

RAD23B

Gene Ontology Biological Process

Gene Ontology Molecular Function

polyubiquitin binding [IDA]protein binding [IPI]single-stranded DNA binding [TAS]

Gene Ontology Cellular Component

CORO1C