BAIT

VPS28

vacuolar protein sorting 28 homolog (S. cerevisiae)
Homo sapiens
PREY

AKTIP

FT1, FTS
AKT interacting protein
UPS Project
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

AKTIP interacts with ESCRT I and is needed for the recruitment of ESCRT III subunits to the midbody.

Merigliano C, Burla R, La Torre M, Del Giudice S, Teo H, Liew CW, Chojnowski A, Goh WI, Olmos Y, Maccaroni K, Giubettini M, Chiolo I, Carlton JG, Raimondo D, Verni F, Stewart CL, Rhodes D, Wright GD, Burke BE, Saggio I

To complete mitosis, the bridge that links the two daughter cells needs to be cleaved. This step is carried out by the endosomal sorting complex required for transport (ESCRT) machinery. AKTIP, a protein discovered to be associated with telomeres and the nuclear membrane in interphase cells, shares sequence similarities with the ESCRT I component TSG101. Here we present evidence that ... [more]

PLoS Genet Dec. 01, 2020; 17(8);e1009757 [Pubmed: 34449766]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
AKTIP VPS28
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID