An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Interactome Rewiring Following Pharmacological Targeting of BET Bromodomains.

Lambert JP, Picaud S, Fujisawa T, Hou H, Savitsky P, Uuskuela-Reimand L, Gupta GD, Abdouni H, Lin ZY, Tucholska M, Knight JDR, Gonzalez-Badillo B, St-Denis N, Newman JA, Stucki M, Pelletier L, Bandeira N, Wilson MD, Filippakopoulos P, Gingras AC

Targeting bromodomains (BRDs) of the bromo-and-extra-terminal (BET) family offers opportunities for therapeutic intervention in cancer and other diseases. Here, we profile the interactomes of BRD2, BRD3, BRD4, and BRDT following treatment with the pan-BET BRD inhibitor JQ1, revealing broad rewiring of the interaction landscape, with three distinct classes of behavior for the 603 unique interactors identified. A group of proteins ... [more]

Mol. Cell Dec. 07, 2018; 73(3);621-638.e17 [Pubmed: 30554943]

Throughput

High Throughput

Additional Notes

FLAG AP-MS dataset using FLAG-tagged BET bait proteins (Table S2A)
High confidence interactions have a Bayesian FDR=<1%
Untreated sample

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
BRD3 XRCC1	Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.	Lambert JP (2019)	High	-	BioGRID	3324965

Curated By

BioGRID

Interaction Summary

BRD3

Gene Ontology Biological Process

Gene Ontology Molecular Function

chromatin binding [IDA]lysine-acetylated histone binding [IDA]protein binding [IPI]

XRCC1

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]

Gene Ontology Cellular Component

Affinity Capture-MS