Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Voltage-dependent conformational changes of Kv1.3 channels activate cell proliferation.

Cidad P, Alonso E, Arevalo-Martinez M, Calvo E, de la Fuente MA, Perez-Garcia MT, Lopez-Lopez JR

The voltage-dependent potassium channel Kv1.3 has been implicated in proliferation in many cell types, based on the observation that Kv1.3 blockers inhibited proliferation. By modulating membrane potential, cell volume, and/or Ca2+ influx, K+  channels can influence cell cycle progression. Also, noncanonical channel functions could contribute to modulate cell proliferation independent of K+ efflux. The specificity of the requirement of Kv1.3 channels for ... [more]

J Cell Physiol Dec. 01, 2020; 236(6);4330-4347 [Pubmed: 33230847]

Throughput

  • High Throughput

Additional Notes

  • Affinity Capture-MS was carried out to identify high confidence protein-protein interactions.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
KCNA3 KCNA3
FRET
FRET

An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins.

Low-BioGRID
-

Curated By

  • BioGRID