An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Interactome Rewiring Following Pharmacological Targeting of BET Bromodomains.

Lambert JP, Picaud S, Fujisawa T, Hou H, Savitsky P, Uuskuela-Reimand L, Gupta GD, Abdouni H, Lin ZY, Tucholska M, Knight JDR, Gonzalez-Badillo B, St-Denis N, Newman JA, Stucki M, Pelletier L, Bandeira N, Wilson MD, Filippakopoulos P, Gingras AC

Targeting bromodomains (BRDs) of the bromo-and-extra-terminal (BET) family offers opportunities for therapeutic intervention in cancer and other diseases. Here, we profile the interactomes of BRD2, BRD3, BRD4, and BRDT following treatment with the pan-BET BRD inhibitor JQ1, revealing broad rewiring of the interaction landscape, with three distinct classes of behavior for the 603 unique interactors identified. A group of proteins ... [more]

Mol. Cell Dec. 07, 2018; 73(3);621-638.e17 [Pubmed: 30554943]

Throughput

High Throughput

Additional Notes

AP-MS with a recombinant BRD4-ET domain identified 151 high-confidence interactors.

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
BRD4 HNRNPH2	Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.	Zhai Y (2022)	High	-	BioGRID	3458394

Curated By

BioGRID

Interaction Summary

BRD4

Gene Ontology Biological Process

Gene Ontology Molecular Function

chromatin binding [IDA]lysine-acetylated histone binding [IDA]p53 binding [IDA]protein binding [IPI]

Gene Ontology Cellular Component

HNRNPH2

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA binding [TAS]poly(A) RNA binding [IDA]