Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

The periphilin 1-like BFAR isoform 3 is highly expressed in transcriptionally silent oocytes and involved in RNA metabolism.

Mrowiec K, Chadalski M, Paszek A, Vydra N, Janus P, Pietrowska M, Toma-Jonik A, Gramatyka M, Widlak W

The mouse 3110001I22Rik gene located in the first intron of Bfar is considered as a Bfar variant coding for the BFARv3 protein. However, it differs from other BFAR isoforms and resembles periphilin 1 (PPHLN1) due to its two (Lge1 and serine-rich) conserved domains. We identified the BFARv3/EGFP-interacting proteins by co-immunoprecipitation coupled to mass spectrometry, which revealed 40S ribosomal proteins (RPS3, ... [more]

Biochim Biophys Acta Mol Cell Res Dec. 01, 2020; 1868(10);119086 [Pubmed: 34175335]

Throughput

  • Low Throughput

Curated By

  • BioGRID