BAIT

CUL4A

RP11-391H12.1

cullin 4A

Fanconi Anemia Project

GO Process (4)

GO Function (1)

GO Component (2)

Gene Ontology Biological Process

G1/S transition of mitotic cell cycle [TAS]

cell cycle arrest [TAS]

intrinsic apoptotic signaling pathway [TAS]

negative regulation of cell proliferation [TAS]

Gene Ontology Molecular Function

protein binding [IPI]

Gene Ontology Cellular Component

Cul4-RING E3 ubiquitin ligase complex [IDA]

Cul4A-RING E3 ubiquitin ligase complex [IDA]

CRISPR Database HGNC Alliance of Genome Resources OMIM Entrez Gene RefSeq UniprotKB Ensembl

Homo sapiens

PREY

UBE2D1

E2(17)KB1, SFT, UBC4/5, UBCH5, UBCH5A

ubiquitin-conjugating enzyme E2D 1

Cell Cycle - Senescence Project

GO Process (24)

GO Function (3)

GO Component (5)

Gene Ontology Biological Process

BMP signaling pathway [TAS]

MyD88-independent toll-like receptor signaling pathway [TAS]

TRIF-dependent toll-like receptor signaling pathway [TAS]

anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process [TAS]

cellular response to hypoxia [TAS]

gene expression [TAS]

innate immune response [TAS]

mitotic cell cycle [TAS]

mitotic spindle assembly checkpoint [TAS]

negative regulation of transcription from RNA polymerase II promoter [TAS]

negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle [TAS]

positive regulation of protein ubiquitination [IDA]

positive regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle [TAS]

protein K48-linked ubiquitination [IDA]

protein polyubiquitination [IDA]

regulation of transcription from RNA polymerase II promoter in response to hypoxia [TAS]

regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle [TAS]

toll-like receptor 3 signaling pathway [TAS]

toll-like receptor 4 signaling pathway [TAS]

toll-like receptor signaling pathway [TAS]

transcription initiation from RNA polymerase II promoter [TAS]

transcription, DNA-templated [TAS]

transforming growth factor beta receptor signaling pathway [TAS]

ubiquitin-dependent protein catabolic process [IDA]

Gene Ontology Molecular Function

protein binding [IPI]
ubiquitin conjugating enzyme activity [IDA]
ubiquitin-protein transferase activity [IDA]

Gene Ontology Cellular Component

cytoplasm [IDA]

nucleoplasm [TAS]

protein complex [IDA]

ubiquitin ligase complex [IDA]

CRISPR Database HGNC Alliance of Genome Resources OMIM Entrez Gene RefSeq UniprotKB Ensembl

Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

BCAS3 exhibits oncogenic properties by promoting CRL4A-mediated ubiquitination of p53 in breast cancer.

Zhou Z, Qiu R, Liu W, Yang T, Li G, Huang W, Teng X, Yang Y, Yu H, Yang Y, Wang Y

Breast cancer-amplified sequence 3 (BCAS3) was initially found to be amplified in human breast cancer (BRCA); however, there has been little consensus on the functions of BCAS3 in breast tumours.We analysed BCAS3 expression in BRCA using bio-information tools. Affinity purification and mass spectrometry were employed to identify BCAS3-associated proteins. GST pull-down and ubiquitination assays were performed to analyse the interaction ... [more]

Cell Prolif Aug. 01, 2021; 54(8);e13088 [Pubmed: 34240781]

Throughput

Low Throughput

Curated By

BioGRID