BAIT

PORCN

DHOF, FODH, MG61, PORC, PPN
porcupine homolog (Drosophila)
GO Process (0)
GO Function (0)
GO Component (0)
Homo sapiens
PREY

WLS

C1orf139, EVI, GPR177, MRP, mig-14, RP11-518D3.2
wntless Wnt ligand secretion mediator
GO Process (2)
GO Function (2)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

EVI/WLS function is regulated by ubiquitylation and is linked to ER-associated degradation by ERLIN2.

Wolf LM, Lambert AM, Haenlin J, Boutros M

WNT signalling is important for development in all metazoans and is associated with various human diseases. The ubiquitin-proteasome system (UPS) and regulatory endoplasmic reticulum-associated degradation (ERAD) have been implicated in the production of WNT proteins. Here, we investigated how the WNT secretory factor EVI (also known as WLS) is ubiquitylated, recognised by ERAD components and subsequently removed from the secretory ... [more]

J Cell Sci Dec. 15, 2020; 134(16); [Pubmed: 34406391]

Throughput

  • Low Throughput

Curated By

  • BioGRID