Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

The interactome of CLUH reveals its association to SPAG5 and its co-translational proximity to mitochondrial proteins.

Hemono M, Haller A, Chicher J, Duchene AM, Ngondo RP

Mitochondria require thousands of proteins to fulfill their essential function in energy production and other fundamental biological processes. These proteins are mostly encoded by the nuclear genome, translated in the cytoplasm before being imported into the organelle. RNA binding proteins (RBPs) are central players in the regulation of this process by affecting mRNA translation, stability, or localization. CLUH is an ... [more]

BMC Biol Jan. 10, 2022; 20(1);13 [Pubmed: 35012549]

Throughput

  • High Throughput

Additional Notes

  • -BioID
  • -fold change greater than 2 and a FDR < 0.1

Curated By

  • BioGRID