CDC42
Gene Ontology Biological Process
- Fc-gamma receptor signaling pathway involved in phagocytosis [TAS]
- GTP catabolic process [TAS]
- Golgi organization [ISS]
- T cell costimulation [TAS]
- actin cytoskeleton organization [IDA]
- axon guidance [TAS]
- blood coagulation [TAS]
- epidermal growth factor receptor signaling pathway [TAS]
- establishment of Golgi localization [ISS]
- establishment or maintenance of cell polarity [TAS]
- innate immune response [TAS]
- macrophage differentiation [TAS]
- muscle cell differentiation [TAS]
- negative regulation of epidermal growth factor receptor signaling pathway [TAS]
- negative regulation of protein complex assembly [IPI]
- organelle transport along microtubule [ISS]
- positive regulation of cytokinesis [IMP]
- positive regulation of muscle cell differentiation [TAS]
- positive regulation of pseudopodium assembly [IDA]
- positive regulation of substrate adhesion-dependent cell spreading [IDA]
- regulation of attachment of spindle microtubules to kinetochore [IMP]
- regulation of filopodium assembly [IDA]
- regulation of small GTPase mediated signal transduction [TAS]
- small GTPase mediated signal transduction [TAS]
- substantia nigra development [IEP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Golgi membrane [ISS]
- cytoplasm [IDA]
- cytoplasmic ribonucleoprotein granule [IDA]
- cytosol [TAS]
- extracellular vesicular exosome [IDA]
- filopodium [IDA]
- focal adhesion [IDA]
- membrane [IDA]
- midbody [IDA]
- mitotic spindle [IDA]
- neuron projection [IDA]
- neuronal cell body [IDA]
- plasma membrane [IDA, TAS]
- spindle midzone [IDA]
ARHGAP32
Gene Ontology Biological Process
Gene Ontology Molecular Function
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Mapping the proximity interaction network of the Rho-family GTPases reveals signalling pathways and regulatory mechanisms.
Guanine nucleotide exchange factors (RhoGEFs) and GTPase-activating proteins (RhoGAPs) coordinate the activation state of the Rho family of GTPases for binding to effectors. Here, we exploited proximity-dependent biotinylation to systematically define the Rho family proximity interaction network from 28 baits to produce 9,939 high-confidence proximity interactions in two cell lines. Exploiting the nucleotide states of Rho GTPases, we revealed the ... [more]
Throughput
- High Throughput|Low Throughput
Additional Notes
- AvgP greater or equal to 0.95 assessed by SAINT express
- BioID
- CDC42 WT bait, interaction in HEK293 cells
- CDC42 wild type bait, interaction in Hela cells
- CDC42-G12V active mutant bait, interaction in HEK293 cells
- CDC42-G12V active mutant bait, interaction in Hela cells
- CDC42-G15A Nucleotide Free mutant bait, interaction in HEK293 cells
- CDC42-G15A nucleotide free mutant bait, interaction in Hela cells
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
ARHGAP32 CDC42 | Biochemical Activity Biochemical Activity An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation. | Low | - | BioGRID | 283299 | |
ARHGAP32 CDC42 | Biochemical Activity Biochemical Activity An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation. | Low | - | BioGRID | 256204 | |
ARHGAP32 CDC42 | Biochemical Activity Biochemical Activity An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation. | Low | - | BioGRID | 256683 | |
CDC42 ARHGAP32 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | - |
Curated By
- BioGRID