BAIT
CDC42
CDC42Hs, G25K, RP1-224A6.5
cell division cycle 42
GO Process (25)
GO Function (7)
GO Component (14)
Gene Ontology Biological Process
- Fc-gamma receptor signaling pathway involved in phagocytosis [TAS]
- GTP catabolic process [TAS]
- Golgi organization [ISS]
- T cell costimulation [TAS]
- actin cytoskeleton organization [IDA]
- axon guidance [TAS]
- blood coagulation [TAS]
- epidermal growth factor receptor signaling pathway [TAS]
- establishment of Golgi localization [ISS]
- establishment or maintenance of cell polarity [TAS]
- innate immune response [TAS]
- macrophage differentiation [TAS]
- muscle cell differentiation [TAS]
- negative regulation of epidermal growth factor receptor signaling pathway [TAS]
- negative regulation of protein complex assembly [IPI]
- organelle transport along microtubule [ISS]
- positive regulation of cytokinesis [IMP]
- positive regulation of muscle cell differentiation [TAS]
- positive regulation of pseudopodium assembly [IDA]
- positive regulation of substrate adhesion-dependent cell spreading [IDA]
- regulation of attachment of spindle microtubules to kinetochore [IMP]
- regulation of filopodium assembly [IDA]
- regulation of small GTPase mediated signal transduction [TAS]
- small GTPase mediated signal transduction [TAS]
- substantia nigra development [IEP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Golgi membrane [ISS]
- cytoplasm [IDA]
- cytoplasmic ribonucleoprotein granule [IDA]
- cytosol [TAS]
- extracellular vesicular exosome [IDA]
- filopodium [IDA]
- focal adhesion [IDA]
- membrane [IDA]
- midbody [IDA]
- mitotic spindle [IDA]
- neuron projection [IDA]
- neuronal cell body [IDA]
- plasma membrane [IDA, TAS]
- spindle midzone [IDA]
Homo sapiens
PREY
DLG1
DLGH1, SAP-97, SAP97, dJ1061C18.1.1, hdlg
discs, large homolog 1 (Drosophila)
GO Process (17)
GO Function (11)
GO Component (17)
Gene Ontology Biological Process
- actin filament organization [IDA]
- axon guidance [TAS]
- cortical actin cytoskeleton organization [IDA]
- dephosphorylation [TAS]
- endothelial cell proliferation [IDA]
- establishment or maintenance of cell polarity [TAS]
- mitotic cell cycle checkpoint [NAS]
- negative regulation of mitotic cell cycle [IMP]
- nucleotide phosphorylation [TAS]
- positive regulation of establishment of protein localization to plasma membrane [IDA]
- positive regulation of potassium ion transport [IDA]
- protein localization to plasma membrane [IMP, TAS]
- regulation of membrane potential [IDA]
- regulation of sodium ion transmembrane transport [TAS]
- single organismal cell-cell adhesion [IDA]
- synaptic transmission [TAS]
- tight junction assembly [IDA]
Gene Ontology Molecular Function- L27 domain binding [IPI]
- cytoskeletal protein binding [TAS]
- guanylate kinase activity [TAS]
- ion channel binding [IPI]
- mitogen-activated protein kinase kinase binding [IPI]
- phosphatase binding [IPI]
- phosphoprotein phosphatase activity [TAS]
- potassium channel regulator activity [IDA, NAS]
- protein C-terminus binding [IPI]
- protein binding [IPI]
- protein kinase binding [IPI]
- L27 domain binding [IPI]
- cytoskeletal protein binding [TAS]
- guanylate kinase activity [TAS]
- ion channel binding [IPI]
- mitogen-activated protein kinase kinase binding [IPI]
- phosphatase binding [IPI]
- phosphoprotein phosphatase activity [TAS]
- potassium channel regulator activity [IDA, NAS]
- protein C-terminus binding [IPI]
- protein binding [IPI]
- protein kinase binding [IPI]
Gene Ontology Cellular Component
- Golgi apparatus [IDA]
- MPP7-DLG1-LIN7 complex [IDA]
- basolateral plasma membrane [IDA]
- cell junction [IDA]
- cell-cell junction [IDA]
- cytoplasm [IDA]
- cytoplasmic side of plasma membrane [IDA]
- cytosol [TAS]
- endoplasmic reticulum [IDA]
- extracellular vesicular exosome [IDA]
- immunological synapse [TAS]
- intercalated disc [TAS]
- microtubule [IDA]
- nucleus [IDA]
- perinuclear region of cytoplasm [IDA]
- plasma membrane [TAS]
- tight junction [IDA]
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Mapping the proximity interaction network of the Rho-family GTPases reveals signalling pathways and regulatory mechanisms.
Guanine nucleotide exchange factors (RhoGEFs) and GTPase-activating proteins (RhoGAPs) coordinate the activation state of the Rho family of GTPases for binding to effectors. Here, we exploited proximity-dependent biotinylation to systematically define the Rho family proximity interaction network from 28 baits to produce 9,939 high-confidence proximity interactions in two cell lines. Exploiting the nucleotide states of Rho GTPases, we revealed the ... [more]
Nat. Cell Biol. Dec. 23, 2019; (); [Pubmed: 31871319]
Throughput
- High Throughput|Low Throughput
Additional Notes
- AvgP greater or equal to 0.95 assessed by SAINT express
- BioID
- CDC42 wild type bait, interaction in Hela cells
- CDC42-G12V active mutant bait, interaction in HEK293 cells
- CDC42-G12V active mutant bait, interaction in Hela cells
- CDC42-G15A nucleotide free mutant bait, interaction in Hela cells
Curated By
- BioGRID