An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Mapping the proximity interaction network of the Rho-family GTPases reveals signalling pathways and regulatory mechanisms.

Bagci H, Sriskandarajah N, Robert A, Boulais J, Elkholi IE, Tran V, Lin ZY, Thibault MP, Dube N, Faubert D, Hipfner DR, Gingras AC, Cote JF

Guanine nucleotide exchange factors (RhoGEFs) and GTPase-activating proteins (RhoGAPs) coordinate the activation state of the Rho family of GTPases for binding to effectors. Here, we exploited proximity-dependent biotinylation to systematically define the Rho family proximity interaction network from 28 baits to produce 9,939 high-confidence proximity interactions in two cell lines. Exploiting the nucleotide states of Rho GTPases, we revealed the ... [more]

Nat. Cell Biol. Dec. 23, 2019; (); [Pubmed: 31871319]

Throughput

High Throughput|Low Throughput

Additional Notes

AvgP greater or equal to 0.95 assessed by SAINT express
BioID
RAC1-G12V active mutant bait, interaction in HEK293 cells
RAC1-G12V active mutant bait, interaction in Hela cells
RAC1-G15A Nucleotide Free mutant bait, interaction in HEK293 cells
RAC1-G15A nucleotide free mutant bait, interaction in Hela cells

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
PAK1 RAC1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Edlund S (2004)	Low	-	BioGRID	-
PAK1 RAC1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Eller-Borges R (2015)	Low	-	BioGRID	-
PAK1 RAC1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Feng H (2015)	Low	-	BioGRID	-
PAK1 RAC1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Jing J (2009)	Low	-	BioGRID	-
PAK1 RAC1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Matos P (2006)	Low	-	BioGRID	-
PAK1 RAC1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Neri P (2011)	Low	-	BioGRID	611322
PAK1 RAC1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Pop M (2004)	Low	-	BioGRID	-
PAK1 RAC1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Qu X (2005)	Low	-	BioGRID	-
PAK1 RAC1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Wells CD (2006)	Low	-	BioGRID	-
PAK1 RAC1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Wu Y (2005)	Low	-	BioGRID	658687
PAK1 RAC1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Yamauchi J (2008)	Low	-	BioGRID	-
PAK1 RAC1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Akakura S (2005)	Low	-	BioGRID	-
PAK1 RAC1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Cheung HN (2014)	Low	-	BioGRID	2658996
PAK1 RAC1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Gonzalez-Martinez D (2004)	Low	-	BioGRID	2327670
PAK1 RAC1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Higashi M (2009)	Low	-	BioGRID	-
PAK1 RAC1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Hou Y (2004)	Low	-	BioGRID	-
PAK1 RAC1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Katoh H (2003)	Low	-	BioGRID	-
PAK1 RAC1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Li W (2018)	Low	-	BioGRID	-
PAK1 RAC1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Miki H (2000)	Low	-	BioGRID	-
PAK1 RAC1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Rocca DL (2015)	Low	-	BioGRID	-
PAK1 RAC1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Sandrock K (2010)	Low	-	BioGRID	1103885
PAK1 RAC1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Seoh ML (2003)	Low	-	BioGRID	-
PAK1 RAC1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Turner LJ (2004)	Low	-	BioGRID	-
RAC1 PAK1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Zhang B (1998)	Low	-	BioGRID	-
RAC1 PAK1	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Bandyopadhyay S (2010)	High	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

RAC1

Gene Ontology Biological Process

Gene Ontology Molecular Function

GTP binding [IDA, IMP]GTPase activity [TAS]Rho GDP-dissociation inhibitor binding [ISS]enzyme binding [IPI]protein binding [IPI]thioesterase binding [IPI]

Gene Ontology Cellular Component

PAK1

Gene Ontology Biological Process

Gene Ontology Molecular Function

collagen binding [IPI]protein binding [IPI]protein kinase activity [IDA]protein serine/threonine kinase activity [IDA, TAS]receptor signaling protein serine/threonine kinase activity [IBA]