An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Mapping the proximity interaction network of the Rho-family GTPases reveals signalling pathways and regulatory mechanisms.

Bagci H, Sriskandarajah N, Robert A, Boulais J, Elkholi IE, Tran V, Lin ZY, Thibault MP, Dube N, Faubert D, Hipfner DR, Gingras AC, Cote JF

Guanine nucleotide exchange factors (RhoGEFs) and GTPase-activating proteins (RhoGAPs) coordinate the activation state of the Rho family of GTPases for binding to effectors. Here, we exploited proximity-dependent biotinylation to systematically define the Rho family proximity interaction network from 28 baits to produce 9,939 high-confidence proximity interactions in two cell lines. Exploiting the nucleotide states of Rho GTPases, we revealed the ... [more]

Nat. Cell Biol. Dec. 23, 2019; (); [Pubmed: 31871319]

Throughput

High Throughput|Low Throughput

Additional Notes

AvgP greater or equal to 0.95 assessed by SAINT express
BioID
RAC2-G12V active mutant bait, interaction in HEK293 cells
RAC2-G12V active mutant bait, interaction in Hela cells

Curated By

BioGRID

Interaction Summary

RAC2

Gene Ontology Biological Process

Gene Ontology Molecular Function

GTP binding [IMP]

Gene Ontology Cellular Component

YKT6