BAIT
RAC3
ras-related C3 botulinum toxin substrate 3 (rho family, small GTP binding protein Rac3)
GO Process (8)
GO Function (3)
GO Component (10)
Gene Ontology Biological Process
- GTP catabolic process [IDA]
- actin cytoskeleton organization [IDA]
- cell projection assembly [IDA]
- intracellular signal transduction [TAS]
- positive regulation of cell adhesion mediated by integrin [IDA]
- positive regulation of substrate adhesion-dependent cell spreading [IDA]
- regulation of small GTPase mediated signal transduction [TAS]
- small GTPase mediated signal transduction [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
CXADR
CAR, CAR4/6, HCAR
coxsackie virus and adenovirus receptor
GO Process (17)
GO Function (8)
GO Component (19)
Gene Ontology Biological Process
- AV node cell to bundle of His cell communication [ISS]
- actin cytoskeleton reorganization [IDA]
- blood coagulation [TAS]
- cardiac muscle fiber development [ISS]
- cell-cell junction organization [ISS]
- defense response to virus [IDA]
- epithelial structure maintenance [IMP]
- gamma-delta T cell activation [ISS]
- germ cell migration [ISS]
- heart development [ISS]
- heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules [IDA]
- homotypic cell-cell adhesion [IDA]
- leukocyte migration [TAS]
- mitochondrion organization [ISS]
- neutrophil chemotaxis [IMP]
- regulation of immune response [TAS]
- transepithelial transport [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- acrosomal vesicle [ISS]
- adherens junction [IDA]
- apicolateral plasma membrane [IDA]
- basolateral plasma membrane [IDA]
- cell body [ISS]
- cell junction [IDA]
- cell-cell junction [IDA]
- extracellular space [IDA]
- filopodium [ISS]
- growth cone [ISS]
- integral component of plasma membrane [NAS]
- intercalated disc [IDA]
- membrane raft [IDA]
- neuromuscular junction [IDA]
- neuron projection [ISS]
- nucleoplasm [IDA]
- plasma membrane [IDA, TAS]
- protein complex [IDA]
- tight junction [IDA]
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Mapping the proximity interaction network of the Rho-family GTPases reveals signalling pathways and regulatory mechanisms.
Guanine nucleotide exchange factors (RhoGEFs) and GTPase-activating proteins (RhoGAPs) coordinate the activation state of the Rho family of GTPases for binding to effectors. Here, we exploited proximity-dependent biotinylation to systematically define the Rho family proximity interaction network from 28 baits to produce 9,939 high-confidence proximity interactions in two cell lines. Exploiting the nucleotide states of Rho GTPases, we revealed the ... [more]
Nat. Cell Biol. Dec. 23, 2019; (); [Pubmed: 31871319]
Throughput
- High Throughput|Low Throughput
Additional Notes
- AvgP greater or equal to 0.95 assessed by SAINT express
- BioID
- RAC3-G12V active mutant bait, interaction in HEK293 cells
- RAC3-G12V active mutant bait, interaction in Hela cells
Curated By
- BioGRID