BAIT
RAC3
ras-related C3 botulinum toxin substrate 3 (rho family, small GTP binding protein Rac3)
GO Process (8)
GO Function (3)
GO Component (10)
Gene Ontology Biological Process
- GTP catabolic process [IDA]
- actin cytoskeleton organization [IDA]
- cell projection assembly [IDA]
- intracellular signal transduction [TAS]
- positive regulation of cell adhesion mediated by integrin [IDA]
- positive regulation of substrate adhesion-dependent cell spreading [IDA]
- regulation of small GTPase mediated signal transduction [TAS]
- small GTPase mediated signal transduction [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
PICALM
CALM, CLTH, LAP
phosphatidylinositol binding clathrin assembly protein
GO Process (22)
GO Function (5)
GO Component (12)
Gene Ontology Biological Process
- cargo loading into vesicle [IMP]
- cell proliferation [IMP]
- clathrin coat assembly [IMP]
- clathrin-mediated endocytosis [IMP]
- endosomal transport [IMP]
- iron ion homeostasis [IMP]
- iron ion import into cell [IMP]
- negative regulation of gene expression [IMP]
- negative regulation of metalloendopeptidase activity involved in amyloid precursor protein catabolic process [ISS]
- negative regulation of receptor-mediated endocytosis [IDA]
- positive regulation of aspartic-type endopeptidase activity involved in amyloid precursor protein catabolic process [ISS]
- positive regulation of beta-amyloid formation [IMP]
- positive regulation of neuron death [IMP]
- positive regulation of transcription, DNA-templated [IDA]
- protein complex assembly [TAS]
- receptor internalization [IMP]
- receptor-mediated endocytosis [IDA, ISS]
- regulation of aspartic-type endopeptidase activity involved in amyloid precursor protein catabolic process [IMP]
- regulation of endocytosis [IMP]
- regulation of protein localization [IDA]
- synaptic vesicle maturation [ISS]
- vesicle-mediated transport [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- AP-2 adaptor complex [IDA]
- clathrin coat of coated pit [IDA]
- coated pit [IDA, ISS]
- intracellular membrane-bounded organelle [IDA]
- membrane [IDA]
- neurofibrillary tangle [IMP]
- neuronal cell body [IDA]
- nucleus [IDA]
- perinuclear region of cytoplasm [ISS]
- postsynaptic membrane [ISS]
- presynaptic membrane [ISS]
- vesicle [ISS]
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Mapping the proximity interaction network of the Rho-family GTPases reveals signalling pathways and regulatory mechanisms.
Guanine nucleotide exchange factors (RhoGEFs) and GTPase-activating proteins (RhoGAPs) coordinate the activation state of the Rho family of GTPases for binding to effectors. Here, we exploited proximity-dependent biotinylation to systematically define the Rho family proximity interaction network from 28 baits to produce 9,939 high-confidence proximity interactions in two cell lines. Exploiting the nucleotide states of Rho GTPases, we revealed the ... [more]
Nat. Cell Biol. Dec. 23, 2019; (); [Pubmed: 31871319]
Throughput
- High Throughput|Low Throughput
Additional Notes
- AvgP greater or equal to 0.95 assessed by SAINT express
- BioID
- RAC3-G12V active mutant bait, interaction in HEK293 cells
- RAC3-G12V active mutant bait, interaction in Hela cells
Curated By
- BioGRID