Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Proteomic analysis identifies novel binding partners of BAP1.

Baas R, J van der Wal F, Bleijerveld OB, van Attikum H, Sixma TK

BRCA1-associated protein 1 (BAP1) is a tumor suppressor and its loss can result in mesothelioma, uveal and cutaneous melanoma, clear cell renal cell carcinoma and bladder cancer. BAP1 is a deubiquitinating enzyme of the UCH class that has been implicated in various cellular processes like cell growth, cell cycle progression, ferroptosis, DNA damage response and ER metabolic stress response. ASXL ... [more]

PLoS One Oct. 01, 2021; 16(9);e0257688 [Pubmed: 34591877]

Throughput

  • Low Throughput

Curated By

  • BioGRID