RHOB
Gene Ontology Biological Process
- GTP catabolic process [TAS]
- Rho protein signal transduction [TAS]
- apoptotic process [TAS]
- axon guidance [TAS]
- blood coagulation [TAS]
- cell adhesion [ISS]
- cellular response to hydrogen peroxide [IDA]
- cellular response to ionizing radiation [IDA]
- cytokinesis [IMP]
- endosome to lysosome transport [IDA]
- negative regulation of cell cycle [ISS]
- platelet activation [TAS]
- positive regulation of angiogenesis [ISS]
- positive regulation of apoptotic process [IMP]
- regulation of small GTPase mediated signal transduction [TAS]
- small GTPase mediated signal transduction [TAS]
- transformed cell apoptotic process [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
PHACTR4
Gene Ontology Biological Process
- Rho protein signal transduction [ISS]
- actin cytoskeleton organization [ISS]
- closure of optic fissure [ISS]
- enteric nervous system development [ISS]
- negative regulation of integrin-mediated signaling pathway [ISS]
- neural crest cell migration [ISS]
- neural tube closure [ISS]
- positive regulation of catalytic activity [ISS]
- regulation of cell cycle [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Mapping the proximity interaction network of the Rho-family GTPases reveals signalling pathways and regulatory mechanisms.
Guanine nucleotide exchange factors (RhoGEFs) and GTPase-activating proteins (RhoGAPs) coordinate the activation state of the Rho family of GTPases for binding to effectors. Here, we exploited proximity-dependent biotinylation to systematically define the Rho family proximity interaction network from 28 baits to produce 9,939 high-confidence proximity interactions in two cell lines. Exploiting the nucleotide states of Rho GTPases, we revealed the ... [more]
Throughput
- High Throughput|Low Throughput
Additional Notes
- AvgP greater or equal to 0.95 assessed by SAINT express
- BioID
- RHOB-G14V active mutant bait, interaction in HEK293 cells
- RHOB-G14V active mutant bait, interaction in Hela cells
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| RHOB PHACTR4 | Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods. | High | 18.99 | BioGRID | 3004247 |
Curated By
- BioGRID