BAIT
RHOC
ARH9, ARHC, H9, RHOH9, RP11-426L16.4
ras homolog family member C
GO Process (6)
GO Function (2)
GO Component (4)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
GJA1
AVSD3, CMDR, CX43, GJAL, HLHS1, HSS, ODDD
gap junction protein, alpha 1, 43kDa
GO Process (11)
GO Function (4)
GO Component (11)
Gene Ontology Biological Process
- atrial cardiac muscle cell action potential [TAS]
- cell communication by electrical coupling [IDA]
- cell-cell signaling [TAS]
- gap junction assembly [TAS]
- heart development [TAS]
- ion transmembrane transport [TAS]
- membrane organization [TAS]
- muscle contraction [TAS]
- positive regulation of I-kappaB kinase/NF-kappaB signaling [IMP]
- signal transduction [IMP]
- transport [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Golgi apparatus [ISS]
- Golgi membrane [TAS]
- Golgi-associated vesicle membrane [TAS]
- endoplasmic reticulum membrane [TAS]
- extracellular vesicular exosome [IDA]
- focal adhesion [IDA]
- gap junction [IDA, ISS]
- integral component of plasma membrane [TAS]
- intercalated disc [IDA, ISS]
- membrane raft [ISS]
- plasma membrane [ISS, TAS]
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Mapping the proximity interaction network of the Rho-family GTPases reveals signalling pathways and regulatory mechanisms.
Guanine nucleotide exchange factors (RhoGEFs) and GTPase-activating proteins (RhoGAPs) coordinate the activation state of the Rho family of GTPases for binding to effectors. Here, we exploited proximity-dependent biotinylation to systematically define the Rho family proximity interaction network from 28 baits to produce 9,939 high-confidence proximity interactions in two cell lines. Exploiting the nucleotide states of Rho GTPases, we revealed the ... [more]
Nat. Cell Biol. Dec. 23, 2019; (); [Pubmed: 31871319]
Throughput
- High Throughput
Additional Notes
- AvgP greater or equal to 0.95 assessed by SAINT express
- BioID
- RHOC-G14V active mutant bait, interaction in HEK293 cells
Curated By
- BioGRID