BAIT
RHOH
ARHH, TTF
ras homolog family member H
GO Process (7)
GO Function (5)
GO Component (3)
Gene Ontology Biological Process
- T cell differentiation [NAS]
- negative regulation of I-kappaB kinase/NF-kappaB signaling [IDA]
- negative regulation of catalytic activity [NAS]
- negative regulation of phosphorylation [IDA]
- regulation of small GTPase mediated signal transduction [TAS]
- regulation of transcription, DNA-templated [NAS]
- small GTPase mediated signal transduction [TAS]
Gene Ontology Molecular Function
Homo sapiens
PREY
VCP
ALS14, HEL-220, HEL-S-70, IBMPFD, IBMPFD1, TERA, p97
valosin containing protein
GO Process (18)
GO Function (8)
GO Component (13)
Gene Ontology Biological Process
- DNA repair [NAS]
- ER-associated ubiquitin-dependent protein catabolic process [IDA, IMP, TAS]
- activation of cysteine-type endopeptidase activity involved in apoptotic process [ISS]
- cellular response to DNA damage stimulus [IDA]
- double-strand break repair [IDA]
- endoplasmic reticulum unfolded protein response [TAS]
- establishment of protein localization [TAS]
- positive regulation of Lys63-specific deubiquitinase activity [IDA]
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process [IDA]
- positive regulation of protein K63-linked deubiquitination [IDA]
- positive regulation of protein catabolic process [IDA]
- positive regulation of protein complex assembly [IDA]
- proteasome-mediated ubiquitin-dependent protein catabolic process [NAS]
- protein N-linked glycosylation via asparagine [IMP]
- protein ubiquitination [IDA, NAS]
- regulation of apoptotic process [TAS]
- retrograde protein transport, ER to cytosol [IDA]
- translesion synthesis [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Hrd1p ubiquitin ligase complex [IDA]
- cytoplasm [IDA]
- cytosol [IDA]
- endoplasmic reticulum [IDA]
- endoplasmic reticulum membrane [IDA]
- extracellular vesicular exosome [IDA]
- intracellular membrane-bounded organelle [ISS]
- lipid particle [IDA]
- nucleoplasm [IDA]
- nucleus [IDA, TAS]
- perinuclear region of cytoplasm [IDA]
- proteasome complex [IDA]
- site of double-strand break [IDA]
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Mapping the proximity interaction network of the Rho-family GTPases reveals signalling pathways and regulatory mechanisms.
Guanine nucleotide exchange factors (RhoGEFs) and GTPase-activating proteins (RhoGAPs) coordinate the activation state of the Rho family of GTPases for binding to effectors. Here, we exploited proximity-dependent biotinylation to systematically define the Rho family proximity interaction network from 28 baits to produce 9,939 high-confidence proximity interactions in two cell lines. Exploiting the nucleotide states of Rho GTPases, we revealed the ... [more]
Nat. Cell Biol. Dec. 23, 2019; (); [Pubmed: 31871319]
Throughput
- High Throughput
Additional Notes
- AvgP greater or equal to 0.95 assessed by SAINT express
- BioID
- RHOH wild type bait, interaction in HEK293 cells
- RHOH wild type bait, interaction in Hela cells
Curated By
- BioGRID