RHOH
Gene Ontology Biological Process
- T cell differentiation [NAS]
- negative regulation of I-kappaB kinase/NF-kappaB signaling [IDA]
- negative regulation of catalytic activity [NAS]
- negative regulation of phosphorylation [IDA]
- regulation of small GTPase mediated signal transduction [TAS]
- regulation of transcription, DNA-templated [NAS]
- small GTPase mediated signal transduction [TAS]
Gene Ontology Molecular Function
CTNND1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Mapping the proximity interaction network of the Rho-family GTPases reveals signalling pathways and regulatory mechanisms.
Guanine nucleotide exchange factors (RhoGEFs) and GTPase-activating proteins (RhoGAPs) coordinate the activation state of the Rho family of GTPases for binding to effectors. Here, we exploited proximity-dependent biotinylation to systematically define the Rho family proximity interaction network from 28 baits to produce 9,939 high-confidence proximity interactions in two cell lines. Exploiting the nucleotide states of Rho GTPases, we revealed the ... [more]
Throughput
- High Throughput
Additional Notes
- AvgP greater or equal to 0.95 assessed by SAINT express
- BioID
- RHOH wild type bait, interaction in HEK293 cells
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| RHOH CTNND1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| RHOH CTNND1 | Co-localization Co-localization Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments. | Low | - | BioGRID | - |
Curated By
- BioGRID