BAIT
RHOH
ARHH, TTF
ras homolog family member H
GO Process (7)
GO Function (5)
GO Component (3)
Gene Ontology Biological Process
- T cell differentiation [NAS]
- negative regulation of I-kappaB kinase/NF-kappaB signaling [IDA]
- negative regulation of catalytic activity [NAS]
- negative regulation of phosphorylation [IDA]
- regulation of small GTPase mediated signal transduction [TAS]
- regulation of transcription, DNA-templated [NAS]
- small GTPase mediated signal transduction [TAS]
Gene Ontology Molecular Function
Homo sapiens
PREY
ITGA5
CD49e, FNRA, VLA5A
integrin, alpha 5 (fibronectin receptor, alpha polypeptide)
GO Process (13)
GO Function (3)
GO Component (7)
Gene Ontology Biological Process
- angiogenesis [TAS]
- axon guidance [TAS]
- blood coagulation [TAS]
- cell adhesion [TAS]
- cell-substrate adhesion [IMP]
- endodermal cell differentiation [IMP]
- extracellular matrix organization [TAS]
- heterotypic cell-cell adhesion [IMP]
- leukocyte migration [TAS]
- negative regulation of anoikis [IMP]
- positive regulation of peptidyl-tyrosine phosphorylation [IMP]
- positive regulation of vascular endothelial growth factor receptor signaling pathway [TAS]
- wound healing, spreading of epidermal cells [IEP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Mapping the proximity interaction network of the Rho-family GTPases reveals signalling pathways and regulatory mechanisms.
Guanine nucleotide exchange factors (RhoGEFs) and GTPase-activating proteins (RhoGAPs) coordinate the activation state of the Rho family of GTPases for binding to effectors. Here, we exploited proximity-dependent biotinylation to systematically define the Rho family proximity interaction network from 28 baits to produce 9,939 high-confidence proximity interactions in two cell lines. Exploiting the nucleotide states of Rho GTPases, we revealed the ... [more]
Nat. Cell Biol. Dec. 23, 2019; (); [Pubmed: 31871319]
Throughput
- High Throughput
Additional Notes
- AvgP greater or equal to 0.95 assessed by SAINT express
- BioID
- RHOH wild type bait, interaction in HEK293 cells
- RHOH wild type bait, interaction in Hela cells
Curated By
- BioGRID