BAIT
RAC1
Rac-1, TC-25, p21-Rac1, MIG5
ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1)
GO Process (41)
GO Function (6)
GO Component (10)
Gene Ontology Biological Process
- Fc-epsilon receptor signaling pathway [TAS]
- Fc-gamma receptor signaling pathway involved in phagocytosis [TAS]
- GTP catabolic process [TAS]
- T cell costimulation [TAS]
- actin cytoskeleton organization [IGI]
- actin filament polymerization [TAS]
- anatomical structure morphogenesis [TAS]
- apoptotic signaling pathway [TAS]
- axon guidance [TAS]
- blood coagulation [TAS]
- cell adhesion [TAS]
- cell motility [IDA]
- cell-matrix adhesion [NAS]
- cellular component movement [TAS]
- inflammatory response [TAS]
- innate immune response [TAS]
- intracellular signal transduction [TAS]
- lamellipodium assembly [IMP]
- localization within membrane [IMP]
- negative regulation of interleukin-23 production [IDA]
- negative regulation of receptor-mediated endocytosis [TAS]
- neurotrophin TRK receptor signaling pathway [TAS]
- platelet activation [TAS]
- positive regulation of Rho protein signal transduction [TAS]
- positive regulation of apoptotic process [TAS]
- positive regulation of cell-substrate adhesion [IGI]
- positive regulation of focal adhesion assembly [IDA]
- positive regulation of lamellipodium assembly [IDA, IMP]
- positive regulation of neutrophil chemotaxis [IMP]
- positive regulation of protein phosphorylation [IMP]
- positive regulation of stress fiber assembly [IDA]
- positive regulation of substrate adhesion-dependent cell spreading [IDA]
- regulation of cell migration [IMP]
- regulation of defense response to virus by virus [TAS]
- regulation of hydrogen peroxide metabolic process [TAS]
- regulation of respiratory burst [IDA]
- response to wounding [TAS]
- ruffle organization [IDA, TAS]
- semaphorin-plexin signaling pathway [ISS]
- substrate adhesion-dependent cell spreading [IMP]
- viral process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
SLC9A1
APNH, NHE-1, NHE1, PPP1R143, RP4-633N17.1
solute carrier family 9, subfamily A (NHE1, cation proton antiporter 1), member 1
GO Process (32)
GO Function (9)
GO Component (11)
Gene Ontology Biological Process
- carbohydrate metabolic process [TAS]
- cell migration [TAS]
- cellular response to acidic pH [IDA, ISS]
- cellular response to epinephrine stimulus [IMP]
- cellular response to mechanical stimulus [TAS]
- cellular sodium ion homeostasis [IDA]
- glycosaminoglycan metabolic process [TAS]
- hyaluronan catabolic process [TAS]
- hyaluronan metabolic process [TAS]
- hydrogen ion transmembrane transport [IDA]
- ion transport [TAS]
- maintenance of cell polarity [TAS]
- positive regulation of NFAT protein import into nucleus [IDA]
- positive regulation of calcineurin-NFAT signaling cascade [IDA]
- positive regulation of calcium:sodium antiporter activity [IMP]
- positive regulation of cardiac muscle hypertrophy [IMP]
- positive regulation of the force of heart contraction [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- protein oligomerization [ISS]
- regulation of cardiac muscle cell membrane potential [TAS]
- regulation of cardiac muscle contraction by calcium ion signaling [IMP]
- regulation of focal adhesion assembly [TAS]
- regulation of intracellular pH [IDA]
- regulation of pH [IDA, TAS]
- regulation of stress fiber assembly [TAS]
- regulation of the force of heart contraction by cardiac conduction [IMP]
- response to acidic pH [IDA]
- response to muscle stretch [IMP]
- small molecule metabolic process [TAS]
- sodium ion export [ISS]
- sodium ion import across plasma membrane [IDA]
- transmembrane transport [TAS]
Gene Ontology Molecular Function- calcium-dependent protein binding [IDA]
- phosphatidylinositol-4,5-bisphosphate binding [TAS]
- protein binding [IPI]
- protein binding, bridging [TAS]
- protein complex scaffold [TAS]
- protein phosphatase 2B binding [IDA, IPI]
- sodium:proton antiporter activity [IDA, ISS]
- sodium:proton antiporter activity involved in regulation of cardiac muscle cell membrane potential [TAS]
- solute:proton antiporter activity [TAS]
- calcium-dependent protein binding [IDA]
- phosphatidylinositol-4,5-bisphosphate binding [TAS]
- protein binding [IPI]
- protein binding, bridging [TAS]
- protein complex scaffold [TAS]
- protein phosphatase 2B binding [IDA, IPI]
- sodium:proton antiporter activity [IDA, ISS]
- sodium:proton antiporter activity involved in regulation of cardiac muscle cell membrane potential [TAS]
- solute:proton antiporter activity [TAS]
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Mapping the proximity interaction network of the Rho-family GTPases reveals signalling pathways and regulatory mechanisms.
Guanine nucleotide exchange factors (RhoGEFs) and GTPase-activating proteins (RhoGAPs) coordinate the activation state of the Rho family of GTPases for binding to effectors. Here, we exploited proximity-dependent biotinylation to systematically define the Rho family proximity interaction network from 28 baits to produce 9,939 high-confidence proximity interactions in two cell lines. Exploiting the nucleotide states of Rho GTPases, we revealed the ... [more]
Nat. Cell Biol. Dec. 23, 2019; (); [Pubmed: 31871319]
Throughput
- High Throughput|Low Throughput
Additional Notes
- AvgP greater or equal to 0.95 assessed by SAINT express
- BioID
- RAC1 wild type bait, interaction in Hela cells
- RAC1-G12V active mutant bait, interaction in Hela cells
- RAC1-G15A nucleotide free mutant bait, interaction in Hela cells
Curated By
- BioGRID