BAIT
CDC42
CDC42Hs, G25K, RP1-224A6.5
cell division cycle 42
GO Process (25)
GO Function (7)
GO Component (14)
Gene Ontology Biological Process
- Fc-gamma receptor signaling pathway involved in phagocytosis [TAS]
- GTP catabolic process [TAS]
- Golgi organization [ISS]
- T cell costimulation [TAS]
- actin cytoskeleton organization [IDA]
- axon guidance [TAS]
- blood coagulation [TAS]
- epidermal growth factor receptor signaling pathway [TAS]
- establishment of Golgi localization [ISS]
- establishment or maintenance of cell polarity [TAS]
- innate immune response [TAS]
- macrophage differentiation [TAS]
- muscle cell differentiation [TAS]
- negative regulation of epidermal growth factor receptor signaling pathway [TAS]
- negative regulation of protein complex assembly [IPI]
- organelle transport along microtubule [ISS]
- positive regulation of cytokinesis [IMP]
- positive regulation of muscle cell differentiation [TAS]
- positive regulation of pseudopodium assembly [IDA]
- positive regulation of substrate adhesion-dependent cell spreading [IDA]
- regulation of attachment of spindle microtubules to kinetochore [IMP]
- regulation of filopodium assembly [IDA]
- regulation of small GTPase mediated signal transduction [TAS]
- small GTPase mediated signal transduction [TAS]
- substantia nigra development [IEP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Golgi membrane [ISS]
- cytoplasm [IDA]
- cytoplasmic ribonucleoprotein granule [IDA]
- cytosol [TAS]
- extracellular vesicular exosome [IDA]
- filopodium [IDA]
- focal adhesion [IDA]
- membrane [IDA]
- midbody [IDA]
- mitotic spindle [IDA]
- neuron projection [IDA]
- neuronal cell body [IDA]
- plasma membrane [IDA, TAS]
- spindle midzone [IDA]
Homo sapiens
PREY
SLC11A2
DCT1, DMT1, NRAMP2, OK/SW-cl.20
solute carrier family 11 (proton-coupled divalent metal ion transporter), member 2
GO Process (22)
GO Function (14)
GO Component (19)
Gene Ontology Biological Process
- activation of cysteine-type endopeptidase activity involved in apoptotic process [IDA]
- cadmium ion transmembrane transport [IDA]
- cation transmembrane transport [IGI]
- cellular iron ion homeostasis [TAS]
- cellular response to oxidative stress [IDA]
- cobalt ion transport [IDA]
- copper ion transmembrane transport [IDA]
- copper ion transport [IDA]
- detection of oxygen [IEP]
- ferrous iron import [IDA]
- ferrous iron transport [IDA]
- lead ion transport [IDA]
- manganese ion transmembrane transport [IDA]
- manganese ion transport [IDA]
- multicellular organismal iron ion homeostasis [IMP]
- nickel cation transmembrane transport [IDA]
- nickel cation transport [IDA]
- response to hypoxia [IEP]
- response to iron ion [IEP]
- transmembrane transport [TAS]
- vanadium ion transport [IDA]
- zinc ion transmembrane transport [IDA]
Gene Ontology Molecular Function- cadmium ion transmembrane transporter activity [IDA]
- calcium ion transmembrane transporter activity [IDA]
- chromium ion transmembrane transporter activity [IDA]
- cobalt ion transmembrane transporter activity [IDA]
- copper ion transmembrane transporter activity [IDA]
- ferrous iron transmembrane transporter activity [IDA]
- inorganic cation transmembrane transporter activity [IGI]
- lead ion transmembrane transporter activity [IDA]
- manganese ion transmembrane transporter activity [IDA]
- nickel cation transmembrane transporter activity [IDA]
- protein binding [IPI]
- solute:proton symporter activity [IDA]
- vanadium ion transmembrane transporter activity [IDA]
- zinc ion transmembrane transporter activity [IDA]
- cadmium ion transmembrane transporter activity [IDA]
- calcium ion transmembrane transporter activity [IDA]
- chromium ion transmembrane transporter activity [IDA]
- cobalt ion transmembrane transporter activity [IDA]
- copper ion transmembrane transporter activity [IDA]
- ferrous iron transmembrane transporter activity [IDA]
- inorganic cation transmembrane transporter activity [IGI]
- lead ion transmembrane transporter activity [IDA]
- manganese ion transmembrane transporter activity [IDA]
- nickel cation transmembrane transporter activity [IDA]
- protein binding [IPI]
- solute:proton symporter activity [IDA]
- vanadium ion transmembrane transporter activity [IDA]
- zinc ion transmembrane transporter activity [IDA]
Gene Ontology Cellular Component
- apical part of cell [IDA]
- apical plasma membrane [IDA]
- basal part of cell [IDA]
- cell surface [IDA]
- cytoplasm [IDA]
- cytoplasmic vesicle [IDA]
- early endosome [IDA]
- late endosome [IDA]
- late endosome membrane [IDA]
- lysosomal membrane [IDA]
- lysosome [IDA]
- membrane [IDA]
- nucleus [IDA]
- paraferritin complex [IDA]
- perinuclear region of cytoplasm [IDA]
- plasma membrane [IDA, IMP, TAS]
- recycling endosome [IDA]
- trans-Golgi network [IDA]
- vacuole [IMP]
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Mapping the proximity interaction network of the Rho-family GTPases reveals signalling pathways and regulatory mechanisms.
Guanine nucleotide exchange factors (RhoGEFs) and GTPase-activating proteins (RhoGAPs) coordinate the activation state of the Rho family of GTPases for binding to effectors. Here, we exploited proximity-dependent biotinylation to systematically define the Rho family proximity interaction network from 28 baits to produce 9,939 high-confidence proximity interactions in two cell lines. Exploiting the nucleotide states of Rho GTPases, we revealed the ... [more]
Nat. Cell Biol. Dec. 23, 2019; (); [Pubmed: 31871319]
Throughput
- High Throughput|Low Throughput
Additional Notes
- AvgP greater or equal to 0.95 assessed by SAINT express
- BioID
- CDC42-G12V active mutant bait, interaction in Hela cells
- CDC42-G15A nucleotide free mutant bait, interaction in Hela cells
Curated By
- BioGRID