BAIT
RAC2
EN-7, Gx, HSPC022, p21-Rac2
ras-related C3 botulinum toxin substrate 2 (rho family, small GTP binding protein Rac2)
GO Process (14)
GO Function (1)
GO Component (6)
Gene Ontology Biological Process
- actin filament organization [IMP]
- axon guidance [TAS]
- blood coagulation [TAS]
- lymphocyte aggregation [IMP]
- platelet activation [TAS]
- positive regulation of lamellipodium assembly [IMP]
- positive regulation of neutrophil chemotaxis [IMP]
- regulation of cell-substrate adhesion [IMP]
- regulation of hydrogen peroxide metabolic process [TAS]
- regulation of neutrophil migration [IMP]
- regulation of respiratory burst [IDA]
- regulation of small GTPase mediated signal transduction [TAS]
- signal transduction [TAS]
- small GTPase mediated signal transduction [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
PTPRJ
CD148, DEP1, HPTPeta, R-PTP-ETA, SCC1
protein tyrosine phosphatase, receptor type, J
GO Process (17)
GO Function (9)
GO Component (8)
Gene Ontology Biological Process
- contact inhibition [NAS]
- negative regulation of MAP kinase activity [IDA]
- negative regulation of T cell receptor signaling pathway [IDA, IMP]
- negative regulation of cell growth [IDA]
- negative regulation of cell migration [IDA]
- negative regulation of cell proliferation [IDA]
- negative regulation of epidermal growth factor receptor signaling pathway [IMP]
- negative regulation of platelet-derived growth factor receptor signaling pathway [IDA]
- negative regulation of protein kinase B signaling [IMP]
- negative regulation of vascular permeability [IDA]
- peptidyl-tyrosine dephosphorylation [IDA, IMP]
- platelet-derived growth factor receptor signaling pathway [IMP]
- positive chemotaxis [IDA]
- positive regulation of cell adhesion [IMP]
- positive regulation of focal adhesion assembly [IMP]
- positive regulation of protein kinase B signaling [IMP]
- regulation of cell adhesion [IMP]
Gene Ontology Molecular Function- beta-catenin binding [IPI]
- delta-catenin binding [IPI]
- gamma-catenin binding [IPI]
- mitogen-activated protein kinase binding [IPI]
- phosphatase activity [IDA, IMP]
- platelet-derived growth factor receptor binding [IPI]
- protein binding [IPI]
- protein kinase binding [IPI]
- protein tyrosine phosphatase activity [IDA, IMP]
- beta-catenin binding [IPI]
- delta-catenin binding [IPI]
- gamma-catenin binding [IPI]
- mitogen-activated protein kinase binding [IPI]
- phosphatase activity [IDA, IMP]
- platelet-derived growth factor receptor binding [IPI]
- protein binding [IPI]
- protein kinase binding [IPI]
- protein tyrosine phosphatase activity [IDA, IMP]
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Mapping the proximity interaction network of the Rho-family GTPases reveals signalling pathways and regulatory mechanisms.
Guanine nucleotide exchange factors (RhoGEFs) and GTPase-activating proteins (RhoGAPs) coordinate the activation state of the Rho family of GTPases for binding to effectors. Here, we exploited proximity-dependent biotinylation to systematically define the Rho family proximity interaction network from 28 baits to produce 9,939 high-confidence proximity interactions in two cell lines. Exploiting the nucleotide states of Rho GTPases, we revealed the ... [more]
Nat. Cell Biol. Dec. 23, 2019; (); [Pubmed: 31871319]
Throughput
- Low Throughput
Additional Notes
- AvgP greater or equal to 0.95 assessed by SAINT express
- BioID
- RAC2-G12V active mutant bait, interaction in Hela cells
Curated By
- BioGRID