BAIT
RAC2
EN-7, Gx, HSPC022, p21-Rac2
ras-related C3 botulinum toxin substrate 2 (rho family, small GTP binding protein Rac2)
GO Process (14)
GO Function (1)
GO Component (6)
Gene Ontology Biological Process
- actin filament organization [IMP]
- axon guidance [TAS]
- blood coagulation [TAS]
- lymphocyte aggregation [IMP]
- platelet activation [TAS]
- positive regulation of lamellipodium assembly [IMP]
- positive regulation of neutrophil chemotaxis [IMP]
- regulation of cell-substrate adhesion [IMP]
- regulation of hydrogen peroxide metabolic process [TAS]
- regulation of neutrophil migration [IMP]
- regulation of respiratory burst [IDA]
- regulation of small GTPase mediated signal transduction [TAS]
- signal transduction [TAS]
- small GTPase mediated signal transduction [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
NF2
ACN, BANF, SCH
neurofibromin 2 (merlin)
GO Process (12)
GO Function (1)
GO Component (8)
Gene Ontology Biological Process
- Schwann cell proliferation [IMP]
- actin cytoskeleton organization [IMP]
- negative regulation of DNA replication [IMP]
- negative regulation of JAK-STAT cascade [IDA]
- negative regulation of cell migration [TAS]
- negative regulation of cell proliferation [IDA, IMP]
- negative regulation of cell-cell adhesion [IDA]
- negative regulation of cell-matrix adhesion [TAS]
- negative regulation of tyrosine phosphorylation of Stat3 protein [IDA]
- negative regulation of tyrosine phosphorylation of Stat5 protein [IDA]
- positive regulation of stress fiber assembly [IMP]
- regulation of hippo signaling [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Mapping the proximity interaction network of the Rho-family GTPases reveals signalling pathways and regulatory mechanisms.
Guanine nucleotide exchange factors (RhoGEFs) and GTPase-activating proteins (RhoGAPs) coordinate the activation state of the Rho family of GTPases for binding to effectors. Here, we exploited proximity-dependent biotinylation to systematically define the Rho family proximity interaction network from 28 baits to produce 9,939 high-confidence proximity interactions in two cell lines. Exploiting the nucleotide states of Rho GTPases, we revealed the ... [more]
Nat. Cell Biol. Dec. 23, 2019; (); [Pubmed: 31871319]
Throughput
- Low Throughput
Additional Notes
- AvgP greater or equal to 0.95 assessed by SAINT express
- BioID
- RAC2-G12V active mutant bait, interaction in Hela cells
Curated By
- BioGRID