BAIT
RAC3
ras-related C3 botulinum toxin substrate 3 (rho family, small GTP binding protein Rac3)
GO Process (8)
GO Function (3)
GO Component (10)
Gene Ontology Biological Process
- GTP catabolic process [IDA]
- actin cytoskeleton organization [IDA]
- cell projection assembly [IDA]
- intracellular signal transduction [TAS]
- positive regulation of cell adhesion mediated by integrin [IDA]
- positive regulation of substrate adhesion-dependent cell spreading [IDA]
- regulation of small GTPase mediated signal transduction [TAS]
- small GTPase mediated signal transduction [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
TNFRSF10B
CD262, DR5, KILLER, KILLER/DR5, TRAIL-R2, TRAILR2, TRICK2, TRICK2A, TRICK2B, TRICKB, ZTNFR9, UNQ160/PRO186
tumor necrosis factor receptor superfamily, member 10b
GO Process (12)
GO Function (3)
GO Component (2)
Gene Ontology Biological Process
- activation of NF-kappaB-inducing kinase activity [NAS]
- activation of cysteine-type endopeptidase activity involved in apoptotic process [TAS]
- apoptotic process [NAS, TAS]
- apoptotic signaling pathway [TAS]
- cell surface receptor signaling pathway [TAS]
- cellular response to mechanical stimulus [IEP]
- extrinsic apoptotic signaling pathway via death domain receptors [NAS]
- intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress [IMP]
- positive regulation of I-kappaB kinase/NF-kappaB signaling [IEP]
- regulation of apoptotic process [NAS]
- regulation of extrinsic apoptotic signaling pathway in absence of ligand [TAS]
- response to endoplasmic reticulum stress [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Mapping the proximity interaction network of the Rho-family GTPases reveals signalling pathways and regulatory mechanisms.
Guanine nucleotide exchange factors (RhoGEFs) and GTPase-activating proteins (RhoGAPs) coordinate the activation state of the Rho family of GTPases for binding to effectors. Here, we exploited proximity-dependent biotinylation to systematically define the Rho family proximity interaction network from 28 baits to produce 9,939 high-confidence proximity interactions in two cell lines. Exploiting the nucleotide states of Rho GTPases, we revealed the ... [more]
Nat. Cell Biol. Dec. 23, 2019; (); [Pubmed: 31871319]
Throughput
- Low Throughput
Additional Notes
- AvgP greater or equal to 0.95 assessed by SAINT express
- BioID
- RAC3-G12V active mutant bait, interaction in Hela cells
Curated By
- BioGRID