BAIT
RAC3
ras-related C3 botulinum toxin substrate 3 (rho family, small GTP binding protein Rac3)
GO Process (8)
GO Function (3)
GO Component (10)
Gene Ontology Biological Process
- GTP catabolic process [IDA]
- actin cytoskeleton organization [IDA]
- cell projection assembly [IDA]
- intracellular signal transduction [TAS]
- positive regulation of cell adhesion mediated by integrin [IDA]
- positive regulation of substrate adhesion-dependent cell spreading [IDA]
- regulation of small GTPase mediated signal transduction [TAS]
- small GTPase mediated signal transduction [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
INHBA
EDF, FRP
inhibin, beta A
GO Process (42)
GO Function (8)
GO Component (3)
Gene Ontology Biological Process
- G1/S transition of mitotic cell cycle [IDA]
- SMAD protein signal transduction [IBA]
- activin receptor signaling pathway [IDA]
- cell cycle arrest [IDA]
- cell development [IBA]
- cell differentiation [TAS]
- cell surface receptor signaling pathway [TAS]
- cell-cell signaling [TAS]
- defense response [TAS]
- endodermal cell differentiation [IDA]
- erythrocyte differentiation [NAS]
- extrinsic apoptotic signaling pathway [IDA]
- eyelid development in camera-type eye [ISS]
- hair follicle development [IGI]
- hematopoietic progenitor cell differentiation [IDA]
- hemoglobin biosynthetic process [IDA]
- male gonad development [IGI]
- negative regulation of B cell differentiation [TAS]
- negative regulation of cell cycle [IDA]
- negative regulation of cell growth [IDA]
- negative regulation of cell proliferation [IDA]
- negative regulation of follicle-stimulating hormone secretion [NAS]
- negative regulation of interferon-gamma biosynthetic process [TAS]
- negative regulation of macrophage differentiation [TAS]
- negative regulation of phosphorylation [TAS]
- nervous system development [NAS]
- odontogenesis [IGI]
- ovarian follicle development [IGI, NAS]
- palate development [IGI]
- positive regulation of cellular protein metabolic process [IDA]
- positive regulation of erythrocyte differentiation [IDA]
- positive regulation of extrinsic apoptotic signaling pathway in absence of ligand [IDA]
- positive regulation of follicle-stimulating hormone secretion [TAS]
- positive regulation of ovulation [ISS]
- positive regulation of pathway-restricted SMAD protein phosphorylation [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- positive regulation of transcription, DNA-templated [IDA]
- progesterone secretion [IGI]
- regulation of MAPK cascade [IBA]
- regulation of follicle-stimulating hormone secretion [IGI]
- regulation of transcription from RNA polymerase II promoter [IDA]
- response to drug [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Mapping the proximity interaction network of the Rho-family GTPases reveals signalling pathways and regulatory mechanisms.
Guanine nucleotide exchange factors (RhoGEFs) and GTPase-activating proteins (RhoGAPs) coordinate the activation state of the Rho family of GTPases for binding to effectors. Here, we exploited proximity-dependent biotinylation to systematically define the Rho family proximity interaction network from 28 baits to produce 9,939 high-confidence proximity interactions in two cell lines. Exploiting the nucleotide states of Rho GTPases, we revealed the ... [more]
Nat. Cell Biol. Dec. 23, 2019; (); [Pubmed: 31871319]
Throughput
- Low Throughput
Additional Notes
- AvgP greater or equal to 0.95 assessed by SAINT express
- BioID
- RAC3-G12V active mutant bait, interaction in Hela cells
Curated By
- BioGRID