An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Mapping the proximity interaction network of the Rho-family GTPases reveals signalling pathways and regulatory mechanisms.

Bagci H, Sriskandarajah N, Robert A, Boulais J, Elkholi IE, Tran V, Lin ZY, Thibault MP, Dube N, Faubert D, Hipfner DR, Gingras AC, Cote JF

Guanine nucleotide exchange factors (RhoGEFs) and GTPase-activating proteins (RhoGAPs) coordinate the activation state of the Rho family of GTPases for binding to effectors. Here, we exploited proximity-dependent biotinylation to systematically define the Rho family proximity interaction network from 28 baits to produce 9,939 high-confidence proximity interactions in two cell lines. Exploiting the nucleotide states of Rho GTPases, we revealed the ... [more]

Nat. Cell Biol. Dec. 23, 2019; (); [Pubmed: 31871319]

Throughput

Low Throughput

Additional Notes

AvgP greater or equal to 0.95 assessed by SAINT express
BioID
RHOB-G14V active mutant bait, interaction in Hela cells

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
RHOB SLC26A6	Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.	Go CD (2021)	High	35	BioGRID	3004343

Curated By

BioGRID

Interaction Summary

RHOB

Gene Ontology Biological Process

Gene Ontology Molecular Function

GTP binding [TAS]GTPase activity [TAS]protein binding [IPI]

Gene Ontology Cellular Component

SLC26A6