RHOB
Gene Ontology Biological Process
- GTP catabolic process [TAS]
- Rho protein signal transduction [TAS]
- apoptotic process [TAS]
- axon guidance [TAS]
- blood coagulation [TAS]
- cell adhesion [ISS]
- cellular response to hydrogen peroxide [IDA]
- cellular response to ionizing radiation [IDA]
- cytokinesis [IMP]
- endosome to lysosome transport [IDA]
- negative regulation of cell cycle [ISS]
- platelet activation [TAS]
- positive regulation of angiogenesis [ISS]
- positive regulation of apoptotic process [IMP]
- regulation of small GTPase mediated signal transduction [TAS]
- small GTPase mediated signal transduction [TAS]
- transformed cell apoptotic process [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
VAMP7
Gene Ontology Biological Process
- ER to Golgi vesicle-mediated transport [ISS]
- autophagic vacuole fusion [IMP]
- calcium ion-dependent exocytosis [ISS]
- endocytosis [IBA]
- endosome to lysosome transport [IDA]
- eosinophil degranulation [IMP, ISS]
- exocytosis [IBA]
- membrane organization [TAS]
- natural killer cell degranulation [IMP]
- neutrophil degranulation [IMP, ISS]
- phagocytosis, engulfment [ISS]
- positive regulation of histamine secretion by mast cell [IMP]
- post-Golgi vesicle-mediated transport [TAS]
- vesicle fusion [IDA]
- vesicle-mediated transport [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Golgi apparatus [IDA]
- SNARE complex [IDA, ISS]
- azurophil granule membrane [IDA]
- cytoplasm [IDA]
- endoplasmic reticulum membrane [ISS]
- extracellular vesicular exosome [IDA]
- intracellular membrane-bounded organelle [IDA]
- lamellipodium [IDA]
- late endosome membrane [ISS]
- lysosomal membrane [IDA, ISS, TAS]
- membrane [IDA]
- neuron projection [ISS]
- phagocytic vesicle [ISS]
- plasma membrane [IDA, TAS]
- platelet alpha granule [IDA]
- pseudopodium [IDA]
- secretory granule [IDA]
- secretory granule membrane [IDA]
- trans-Golgi network [IDA]
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Mapping the proximity interaction network of the Rho-family GTPases reveals signalling pathways and regulatory mechanisms.
Guanine nucleotide exchange factors (RhoGEFs) and GTPase-activating proteins (RhoGAPs) coordinate the activation state of the Rho family of GTPases for binding to effectors. Here, we exploited proximity-dependent biotinylation to systematically define the Rho family proximity interaction network from 28 baits to produce 9,939 high-confidence proximity interactions in two cell lines. Exploiting the nucleotide states of Rho GTPases, we revealed the ... [more]
Throughput
- Low Throughput
Additional Notes
- AvgP greater or equal to 0.95 assessed by SAINT express
- BioID
- RHOB-G14V active mutant bait, interaction in Hela cells
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
RHOB VAMP7 | Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods. | High | 30 | BioGRID | 3004421 |
Curated By
- BioGRID