BAIT
RHOV
ARHV, CHP, WRCH2
ras homolog family member V
GO Process (2)
GO Function (1)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
GNAS
AHO, C20orf45, GNAS1, GPSA, GSA, GSP, NESP, PHP1A, PHP1B, PHP1C, POH, RP4-543J19.4
GNAS complex locus
GO Process (29)
GO Function (11)
GO Component (10)
Gene Ontology Biological Process
- activation of adenylate cyclase activity [TAS]
- adenylate cyclase-activating G-protein coupled receptor signaling pathway [IDA, IMP]
- adenylate cyclase-activating adrenergic receptor signaling pathway [IDA]
- adenylate cyclase-activating dopamine receptor signaling pathway [IBA, ISS]
- blood coagulation [TAS]
- bone development [IDA, IMP]
- cAMP biosynthetic process [TAS]
- cellular response to catecholamine stimulus [ISS]
- cellular response to glucagon stimulus [TAS]
- cellular response to prostaglandin E stimulus [ISS]
- cognition [IDA, IMP]
- developmental growth [IDA, IMP]
- energy reserve metabolic process [TAS]
- female pregnancy [NAS]
- hair follicle placode formation [IDA, IMP]
- intracellular transport [NAS]
- negative regulation of multicellular organism growth [ISS]
- platelet aggregation [IDA, IMP]
- positive regulation of Ras GTPase activity [IDA]
- positive regulation of cAMP biosynthetic process [IDA]
- positive regulation of cAMP-mediated signaling [IDA]
- protein secretion [NAS]
- regulation of insulin secretion [TAS]
- response to parathyroid hormone [IMP]
- sensory perception of chemical stimulus [IBA]
- sensory perception of smell [TAS]
- small molecule metabolic process [TAS]
- transmembrane transport [TAS]
- water transport [TAS]
Gene Ontology Molecular Function- D1 dopamine receptor binding [IBA]
- G-protein beta/gamma-subunit complex binding [IBA]
- GTPase activity [IBA, TAS]
- adenylate cyclase activity [TAS]
- beta-2 adrenergic receptor binding [IBA]
- corticotropin-releasing hormone receptor 1 binding [IBA]
- insulin-like growth factor receptor binding [IBA]
- ionotropic glutamate receptor binding [IBA]
- mu-type opioid receptor binding [IBA]
- protein binding [IPI]
- signal transducer activity [IBA, IDA]
- D1 dopamine receptor binding [IBA]
- G-protein beta/gamma-subunit complex binding [IBA]
- GTPase activity [IBA, TAS]
- adenylate cyclase activity [TAS]
- beta-2 adrenergic receptor binding [IBA]
- corticotropin-releasing hormone receptor 1 binding [IBA]
- insulin-like growth factor receptor binding [IBA]
- ionotropic glutamate receptor binding [IBA]
- mu-type opioid receptor binding [IBA]
- protein binding [IPI]
- signal transducer activity [IBA, IDA]
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Mapping the proximity interaction network of the Rho-family GTPases reveals signalling pathways and regulatory mechanisms.
Guanine nucleotide exchange factors (RhoGEFs) and GTPase-activating proteins (RhoGAPs) coordinate the activation state of the Rho family of GTPases for binding to effectors. Here, we exploited proximity-dependent biotinylation to systematically define the Rho family proximity interaction network from 28 baits to produce 9,939 high-confidence proximity interactions in two cell lines. Exploiting the nucleotide states of Rho GTPases, we revealed the ... [more]
Nat. Cell Biol. Dec. 23, 2019; (); [Pubmed: 31871319]
Throughput
- High Throughput
Additional Notes
- AvgP greater or equal to 0.95 assessed by SAINT express
- BioID
- RHOV-G40V active mutant bait, interaction in Hela cells
Curated By
- BioGRID